Dilution or Resuspension
Whether you’re diluting a 100 µM primer down to 10 µM for routine PCR or figuring out how many µL of buffer will turn a 25 nmol lyophilised oligo into a 100 µM stock, the Simple Dilution & Resuspension Calculator gives an instant, error-free answer right in your browser. It wraps the time-honoured C₁V₁ = C₂V₂ equation and the standard oligo-resuspension formula into a clean two-mode widget, so anyone in a wet-lab can move from tube to thermocycler without hunting for a spreadsheet or making slip-ups that ruin a qPCR curve.
Got a 100 µM primer tube but only need 10 µM? Or a lyophilised 25 nmol oligo that must become a 100 µM stock?
This tool uses the classic C1 V1 = C2 V2 relationship to tell you exactly how much stock or buffer to add—no spreadsheets, no slip-ups. The same equation sits at the heart of every lab dilution chart. All calculations run locally in your browser, so your concentrations stay private.
Who is it for?
Bench scientists & technicians
Dilutions are daily bread: every fresh tube of commercial primer arrives at 100 µM and must be stepped down—usually 10-fold—to match PCR protocols.
qPCR & diagnostics labs
Serial ten-fold dilutions underpin standard-curve efficiency; even a ±20 % pipetting error can shift efficiency from 86 % to 119 % and force a complete rerun.
Teaching labs & students
Intro-level courses drill the C₁V₁ = C₂V₂ relationship as the go-to way to connect concentration and volume.
Why it’s useful
- Cuts calculation time from minutes to seconds—no spreadsheet templates, no paper charts.
- Reduces expensive redo’s—wrong dilutions waste reagent and instrument time; a quick check prevents that.
- Browser-only privacy—all math runs client-side, matching Thermo Fisher and IDT practice for proprietary sequences.
- Covers both wet and dry formats—dilution mode for liquids, resuspension mode for lyophilised oligos (nmol → µM).
How it helps in day-to-day lab life
| Scenario | Pain-point | Calculator win |
|---|---|---|
| Primer arrival day | Convert 100 µM tube to 10 µM working stock | Enter 100, 10, and the final volume; tool returns exact µL stock + diluent |
| qPCR standard curve | Need 5×10-fold dilutions with 20 µL each | Set C₁, C₂, V₂ once; repeat for the chain without manual math |
| CRISPR donor prep | Resuspend 1 nmol ssODN to 200 µM | Resuspension mode outputs the single buffer volume—no back-of-envelope |
| Teaching demo | Show C₁V₁ = C₂V₂ in action | Project the widget; students see real-time answers before pipetting |
How it works under the hood
- Dilution mode rearranges C₁V₁ = C₂V₂ to V₁ = (C₂ × V₂)/C₁, then shows both V₁ (stock) and V₂ – V₁ (diluent).
- Resuspension mode uses Omni Calculator’s nmol-to-µM relation: µL = (nmol × 1000) / µM.
- Both paths run in vanilla JavaScript, so there’s zero server lag, zero data capture, and no compliance worries—ideal for clinical or IP-sensitive work.
Tips for confident dilutions
- Pre-wet pipette tips and use reverse-pipetting to cut CV by >50 % in µL-scale transfers.
- Use the widest possible difference between C₁ and C₂ in a single step; multi-step serials multiply pipetting error.
- Label both stock and working tubes with concentration and date—your calculator output gives exact numbers to copy.