Instant Bio-Toolbox: Calculators for Every Cloning or Protein Workflow
GC-content Calculator
Copy-Number ↔ Molarity
Primer Tm & Oligo Analyzer
Dilution & Resuspension
Codon-Optimisation Wizard
Restriction-Site Mapper
DNA → Protein Translator
Protein Mw / pI
Ligation Ratio Helper
Reverse Complement Formatter
Every wet-lab workflow—from the first PCR to the final protein gel—leans on quick, low-friction maths. Each tool below runs client-side, so sequences and concentrations never leave your browser. All follow the same card style, making them easy to scan, embed, or bookmark.
What each calculator does
1 · GC-content Calculator
- For: students checking primer bias; NGS techs flagging low-GC regions.
- Does: counts bases, outputs %GC in milliseconds.
- Benefit: predicts PCR success and Illumina coverage dips.
2 · Copy-Number ↔ Molarity Converter
- Thermo Fisher’s own web tool is a daily reference for qPCR prep.
- Our clone keeps data private while matching the same constants.
3 · Primer Tm & Oligo Analyzer
- NEB and IDT call Tm the critical design parameter.
- Flags hairpins & dimers to cut failed PCRs before they happen.
4 · Simple Dilution & Resuspension
- Mirrors Omni Calculator’s C1V1=C2V2 form.
- Nests two modes: liquid dilutions and nmol→µM resuspension.
5 · Codon-Optimisation Wizard
- VectorBuilder & GenScript show codon swaps boost expression 2-10×.
- Ideal for iGEM teams expressing human genes in E. coli.
6 · Restriction-Site Mapper
- GenScript’s map app is still the fastest cloning sanity check.
- This version runs offline—good for IP-sensitive plasmids.
7 · DNA → Protein Translator
- Expasy’s translator is a classroom staple.
- Three frames side-by-side teach stop-codon spotting instantly.
8 · Protein Mw / pI Calculator
- Bioinformatics.org & ExPASy ProtParam use the same residue masses.
- Helps pick SDS-PAGE markers and ion-exchange buffer pH.
9 · Insert-to-Vector Ligation Ratio Helper
- Addgene insists on 3 : 1 molar ratios in its protocols.
- Formula: (Insert bp / Vector bp) × Ratio × Vector ng → Insert ng.
- Saves cloning evenings from self-ligation failures.
10 · One-click Reverse-Complement & Sequence Formatter
- For: bench scientists flipping strands; educators demoing transcription; bioinformaticians fixing FASTA wrap.
- Does: one-click reverse-complement, U ↔ T swap, case toggle, line-length wrap/unwrap.
- Benefit: eliminates clipboard gymnastics and keeps sequences offline, speeding every primer or FASTA tweak.
Quick reference tables for students
- Typical molar ratios
Sticky ends: 3 : 1 → 10 : 1 • Blunt ends: 5 : 1 → 10 : 1
(NEBioCalculator presets) - Common enzyme recognition sites (EcoRI GAATTC, BamHI GGATCC…)—use the Restriction-Site Mapper card above for the full list.
With every calculator in one scroll, students, technicians, and start-ups get an end-to-end digital bench companion—all without leaving EFB Public.