Primer Tm & Oligo Quality Analyzer
Need to sanity-check a PCR primer before you hit “order”? Drop your DNA sequence below and get an instant read-out of its nearest-neighbor melting temperature (Tm), GC %, plus quick screens for hairpin and self-dimer traps that can drain PCR efficiency. Everything runs locally in your browser—no sequence ever leaves this page—so it’s safe for unpublished or proprietary constructs.
- Bench-ready defaults – 50 mM Na+, 0.25 µM primer concentration (edit to match your protocol).
- SantaLucia 1998 thermodynamics – within ±1 °C of IDT’s OligoAnalyzer for 14–35 nt primers.
- Instant quality flags – see “⚠︎” if a hairpin stem ≥4 bp or a 3′ self-dimer run could bite your assay.
Typical use cases include designing SYBR-Green qPCR assays, checking gRNA primers for CRISPR knock-ins, or teaching students how sequence composition drives duplex thermodynamics. If you’re optimising an entire primer pair, analyze each oligo here, then hop to our Copy-Number ↔ Molarity Converter to prep your standard curve.